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KMID : 1101420150470020083
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Korean Journal of Clinical Laboratory Science
2015 Volume.47 No. 2 p.83 ~ p.89
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Identification of Mycobacteria Using Polymerase Chain Reaction and Sputum Sample
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Jang Hyung-Seok
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Abstract
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Although Mycobacterium tuberculosis complex strains remain responsible for the majority of diseases caused by mycobacterial infections worldwide, the increase in HIV (human immuno deficiency virus) infections has allowed for the emergence of other non-tuberculous mycobacteria as clinically significant pathogens. M. tuberculosis was detected by two-tube nested polymerase chain reaction (PCR) and non-tuberculous mycobacteria was detected by PCR-restriction fragment length polymorphism (RFLP) with Msp I. Result of niacin test is equal to result of two-tube nested PCR after culture for M. tuberculosis. In this study, acid fast bacilli stain (AFB. stain) >2+ case, Detection of Mycobacteria is similar to result before culture and after culture. AFB. stain <1+ case, result of mycobacteria is distinguished. Conclusionly, these results suggest that identification of mycobacteria must go side by side both culture and PCR for more fast and accuracy.
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KEYWORD
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Mycobacterium tuberculosis, nontuberculous mycobacteria, acid fast bacilli stain, Polymerase chain reaction, PCR-restriction fragment length polymorphism
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